構造生物 Vol.3 No.3
1997年12月発行

Purification Methods for Biomolecule using Perfusion Chromatography


Hiroyuki Fukuda

Nihon PerSeptive Ltd., Minato-ku, Tokyo 106, Japan

Liquid chromatography is a vitally important technology in life science research. The technique provides a unique combination of capabilities. It provides the separation power needed to purify even subtle molecular variants from complex mixtures, combined with gentle chemical and physical conditions which enable recovery ofbiological activity for complex, biological macromolecules. Although the product of a chromatographic separation may be the most important starting point for a research project (e.g. a pure protein for X-ray crystallographic studies), actually performing the chromatography itself is often viewed as the most tedious part of the project. Perfusion Chromatography technology represents a fundamental advance in the design of chromatography particles. POROS Perfusion Chromatography media are designed to radically speed access to the interior of the chromatography particles by overcoming the diffasional mass transfer limitations of conventional chromatographic media. The most important advancement enabled by Perfusion Chromatography is a practical, systematic approach to the development of a separation method, which enable purification of biomolecule in high purity.


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